ほうれん草葉緑体の抗酸化系に対するマグネシウム欠乏とセリウムの影響

ほうれん草培養に適用されるマグネシウム欠損条件は、活性酸素種（ROS）の蓄積の増加によって監視されたほうれん草の葉緑体の酸化ストレス状態を引き起こしました。The enhancement of lipids peroxide of spinach chloroplast grown in magnesium-deficiency media suggested an oxidative attack that was activated by a reduction of antioxidative defense mechanism measured by analysing the activities of superoxide dismutase, catalase, ascorbate peroxidase, guaiacol peroxidase, and glutathione reductase, asカロテノイドなどの抗酸化物質とグルタチオン含有量。葉緑体の抗酸化反応は、マグネシウム欠損培地で成長したほうれん草で減少したため、ほうれん草の植物の重量の大幅な減少を引き起こし、古い葉はクロロシスを回しました。しかし、マグネシウム欠損条件で成長したセリウム治療により、マロンジアルデヒドとROSが減少し、抗酸化防御システムの活性が増加し、ほうれん草の成長が改善されました。一緒に、実験的研究は、セリウムがマグネシウムの代わりに部分的に代用し、マグネシウム欠損条件で成長したほうれん草の葉緑体の酸化ストレス耐性を増加させることができることを暗示していますが、メカニズムにはさらなる研究が必要です。

Magnesium-deficiency conditions applied to spinach cultures caused an oxidative stress status in spinach chloroplast monitored by an increase in reactive oxygen species (ROS) accumulation. The enhancement of lipids peroxide of spinach chloroplast grown in magnesium-deficiency media suggested an oxidative attack that was activated by a reduction of antioxidative defense mechanism measured by analysing the activities of superoxide dismutase, catalase, ascorbate peroxidase, guaiacol peroxidase, and glutathione reductase, as well as antioxidants such as carotenoids and glutathione content. As the antioxidative response of chloroplast was reduced in spinach grown in magnesium-deficiency media, it caused a significant reduction of spinach plant weight, old leaves turning chlorosis. However, cerium treatment grown in magnesium-deficiency conditions decreased the malondialdehyde and ROS, and increased activities of the antioxidative defense system, and improved spinach growth. Together, the experimental study implied that cerium could partly substitute for magnesium and increase the oxidative stress-resistance of spinach chloroplast grown in magnesium-deficiency conditions, but the mechanisms need further study.