Mycobacterium Avium Mav2054およびMav5183タンパク質の血清診断の可能性

Mycobacterium avium-m。細胞内複合体（MAC）は、結核（TB）と同様の肺疾患（PD）を引き起こします。MAC-PDの診断は複雑で時間がかかります。この研究では、新たに同定されたMAV2054およびMAV5183タンパク質の血清症の可能性は、MAC-PD、肺結核、または潜在性結核を患っている被験者と、HSPXおよび38-KDAアンチゲンとともに、非感染の健康コントロール（HC）で評価されました。 - 知られている血清診断M.結核抗原。4つの抗原すべてが、潜在性結核およびHC被験者よりもMAC-PDおよび活性結核で有意に高いIgG応答を誘発しました。抗原の中で、MAV2054は肺結核およびMAC-PD患者の最高の抗体反応を引き出しました。MAV2054およびMAV5183に対するIgG力価は、肺結核被験者よりもMAC-PDの方が有意に高かった。さらに、M。intracellulareおよび線維能形態のすべての抗原に対するIgGのレベルは、それぞれM. aviumおよび結節性気管支形態のものよりも高かった。感度と受信機の特性曲線分析に基づいて、MAC-PDおよび肺結核の検出に最適な候補は、それぞれMAV2054と38 kDA抗原でした。合計で、MAC-PDの76.0％と活性結核患者の65.0％が少なくとも2つの抗原に反応しました。対照的に、HC被験者の2.8％のみが2つ以上の抗原と反応していました。我々の発見は、4つの抗原を使用した酵素結合免疫吸着剤アッセイ（ELISA）が、MAC-PDや肺結核を含むマイコバクテリア肺疾患のスクリーニングに役立つことを示唆していますが、疾患を引き起こす病原体の良好な識別を提供しません。

The Mycobacterium avium-M. intracellulare complex (MAC) causes a pulmonary disease (PD) similar to tuberculosis (TB). Diagnosis of MAC-PD is complicated and time-consuming. In this study, the serodiagnostic potential of the newly identified MAV2054 and MAV5183 proteins was evaluated in subjects with MAC-PD, pulmonary TB, or latent TB and in noninfected healthy controls (HC), together with HspX and the 38-kDa antigen, well-known serodiagnostic M. tuberculosis antigens. All four antigens evoked significantly higher IgG responses in MAC-PD and active TB than in latent TB and HC subjects. Among the antigens, MAV2054 elicited the highest antibody responses in pulmonary TB and MAC-PD patients. IgG titers against MAV2054 and MAV5183 were significantly higher in MAC-PD than in pulmonary TB subjects. In addition, the levels of IgG against all antigens in the M. intracellulare and fibrocavitary forms were higher than those in the M. avium and nodular bronchiectatic forms, respectively. Based on sensitivity and receiver operator characteristic curve analysis, the best candidates for detection of MAC-PD and pulmonary TB were MAV2054 and the 38-kDa antigen, respectively. In total, 76.0% of MAC-PD and 65.0% of active TB patients were reactive to at least two antigens. In contrast, only 2.8% of HC subjects were reactive with two or more antigens. Our findings suggest that an enzyme-linked immunosorbent assay (ELISA) using the four antigens would be valuable for screening for mycobacterial lung disease, including MAC-PD and pulmonary TB, although it does not provide good discrimination of the disease-causing pathogens.