CCR7-CD45RA-CD27+ CD28+エフェクターメモリサブセットのCD45RA-FOXP3（低）非規制T細胞は、関節リウマチ患者の滑液で増加します。

末梢血と比較して、関節リウマチ（RASF）患者の滑液（TREG）細胞の増加は滑液で見られます。ただし、RASFのTreg細胞は、T細胞を抑制する能力が低下することが示されています。ここでは、CD45RA、CCR7、CD27、およびCD28の発現に基づいて、RASFのCD4+ T細胞を表現型に分類したCD4+ T細胞を6つのサブセットに分類し、CCR7-CD45RA-CD27+ CD28+ TEMサブセットが末梢血と比較して滑液で有意に増加したことを実証しました。さらに、RASFでは、CCR7-CD45RA-CD27+ CD28+ TEMサブセットのFOXP3+ TREG細胞の割合が有意に増加しました。さらに、RASFのFOXP3+ Treg細胞のほとんどは、非抑制CD45RA-FOXP3（低）非TREG細胞であり、CCR7-CD45RA-CD27+ CD28+ TEMサブセットの非TREG細胞の頻度はRASFで有意に増加しました。。私たちの発見は、RA関節の炎症誘発性環境が滑液中のCD45RA-FOXP3（低）非TREG細胞の増加を誘導する可能性があることを示唆しています。

Increased numbers of regulatory T (Treg) cells are found in synovial fluid from patients with rheumatoid arthritis (RASF) compared with peripheral blood. However, Treg cells in RASF have been shown to have a decreased capacity to suppress T cells. Here we phenotypically classified CD4+ T cells in RASF into six subsets based on the expression of CD45RA, CCR7, CD27 and CD28, and demonstrated that the CCR7-CD45RA-CD27+CD28+ TEM subset was significantly increased in synovial fluid compared with peripheral blood. In addition, the proportion of Foxp3+ Treg cells in the CCR7-CD45RA-CD27+CD28+ TEM subset was significantly increased in RASF. Furthermore, most of the Foxp3+ Treg cells in RASF were non-suppressive CD45RA-Foxp3(low) non-Treg cells, and the frequency of the non-Treg cells in the CCR7-CD45RA-CD27+CD28+ TEM subset was significantly increased in RASF. Our findings suggest that the pro-inflammatory environment in RA joints may induce the increase of CD45RA-Foxp3(low) non-Treg cells in synovial fluid.