好中球顆粒球上のFC-GAMMA受容体III（CD16）およびFC-GAMMA受容体I（CD64）の高発現の低い発現

好中球顆粒球を介した過炎炎症は、Covid-19肺炎の疾患の重症度に寄与し、急性肺不全を促進します。骨髄細胞コンパートメント内の調節不全のメカニズムを理解することは、重度のCOVID-19感染の治療法を改善するのに役立つ可能性があります。ここでは、肺浸潤患者9人と比較して、covid-19のない患者と比較して、covid-19肺炎患者16人の患者の循環好中球顆粒球と単球の免疫病理学的特性を調査しました。免疫表現型を、重症度分類システムのスコアApache-IIと相関させました。CD15の平均蛍光強度（MFI）は、内皮間移動にとって重要であるCD15の平均蛍光強度（MFI）が、COVID-19の患者で大幅に減少したことがわかりました（差異±SD; 295.70±117.50 MFI; P = 0.02）。さらに、COVID-19の患者の好中球顆粒球では顆粒性が有意に低かった（差±SD; 1.11±0.43側散乱比; P = 0.02）。さらに、好中球顆粒球上のFC-GAMMA受容体III（CD16）およびFC-GAMMA受容体I（CD64）は、COVID-19の重症度と不調和的に発現しました。CD16は、反比例の（ρ=（ - ）0.72; 95％CI（ - ）0.92-（ - ）0.23; p = 0.01）と比例する（ρ= 0.76; 95％CI 0.31-0.93; p = 0.01）と相関して相関しています。患者のApache-IIスコアで。FC-GAMMA受容体の逸脱した発現は、COVID-19肺炎の重度の症例における調節不全抗体媒介食作用において役割を果たす可能性があると結論付けています。

Hyperinflammation through neutrophil granulocytes contributes to disease severity in COVID-19 pneumonia and promotes acute lung failure. Understanding the mechanisms of the dysregulations within the myeloid cell compartment may help to improve therapies for severe COVID-19 infection. Here, we investigated the immunopathological characteristics of circulating neutrophil granulocytes and monocytes in 16 patients with COVID-19 pneumonia by multiparameter flow cytometry in comparison to 9 patients with pulmonary infiltrates but without COVID-19. We correlated the immunophenotypes with the scores of the severity-of-disease classification system, APACHE-II. We found that the mean fluorescence intensity (MFI) of CD15, which is important for the transendothelial migration, was significantly reduced in the patients with COVID-19 (difference ± SD; 295.70 ± 117.50 MFI; p = 0.02). In addition, the granularity was significantly lower in the neutrophil granulocytes of patients with COVID-19 (difference ± SD; 1.11 ± 0.43 side-scatter ratio; p = 0.02). Moreover, the Fc-gamma receptor III (CD16) and Fc-gamma receptor I (CD64) on the neutrophil granulocytes were expressed discordantly with COVID-19 severity. CD16 correlated as inversely proportional (ρ = (-)0.72; 95% CI (-)0.92-(-)0.23; p = 0.01) and CD64 as proportional (ρ = 0.76; 95% CI 0.31-0.93; p = 0.01) with the APACHE-II scores of the patients. We conclude that the deviant expression of the Fc-gamma receptors might play role in a dysregulated antibody-mediated phagocytosis in severe cases of COVID-19 pneumonia.