眼外の中空繊維透析装置でのアクリル微粒子における固定化されたL-アスパラギナーゼの使用

L-アスパラギナーゼは、ポリアクリルアミドの球状微粒子の無菌条件下で固定化されました。血液と酵素間の直接接触を避けるために、ヘモフィルターの毛細血管繊維の外面に微粒子に固定化されたL-アスパラギナーゼを適用しました。ヘモフィルターは、in vitroおよびin vivoの両方で、L-アスパラギンのL-アスパラギン酸への変換において非常に効率的でした。緩衝液中のL-アスパラギン（5リットルで50ミクロム）を、2000 I.U.を含むヘモフィルターを介して循環してから60分以内にL-アスパラギン酸に変換されました。L-アスパラギナーゼの。Circulating L-asparagine in healthy sheep (about 40-50 microM was reduced to low levels after 2 to 3 hr of perfusion with a unit containing 2000 I.U. of L-asparaginase. The reduction persisted for 3 to 4 hr after terminated treatment. Repeated, extracorporeal treatments in sheep showed that the L-asparagine decrease induced an increased resynthesis of L-asparagine, probably due to the action of theL-アスパラギンシンテターゼ。

L-asparaginase was immobilized under aseptic conditions in spherical microparticles of polyacrylamide. To avoid direct contact between blood and enzyme, we have applied the immobilized L-asparaginase in microparticles on the outer surface of the capillary fibers of a hemofilter. The hemofilters were very efficient in the transformation of L-asparagine to L-aspartic acid, both in vitro and in vivo. L-Asparagine in buffer (50 microM in 5 liters) was converted to L-aspartic acid within 60 min after circulation through a hemofilter containing 2000 I.U. of L-asparaginase. Circulating L-asparagine in healthy sheep (about 40-50 microM was reduced to low levels after 2 to 3 hr of perfusion with a unit containing 2000 I.U. of L-asparaginase. The reduction persisted for 3 to 4 hr after terminated treatment. Repeated, extracorporeal treatments in sheep showed that the L-asparagine decrease induced an increased resynthesis of L-asparagine, probably due to the action of the L-asparagine synthetase.