ラット脳におけるL-2-アミノ-4-ホスホノブチレート感受性メタボトロピックグルタミン酸受容体、mGluR4およびMGLUR7のmRNAの分布

L-2-アミノ-4-ホスホノブチレート（L-AP4）に非常に敏感な代謝性グルタミン酸受容体MGLUR4およびMGLUR7のmRNAの分布は、in situハイブリダイゼーションによりラットの中枢神経系で調べられました。一般に、mGluR7 mRNAのハイブリダイゼーションシグナルはMGLUR4 mRNAのハイブリダイゼーションシグナルよりも広く分布しており、MGLUR4 mRNAおよびMGLUR7 mRNAの微分発現は、一部の脳領域で明確に示されていました。嗅球と小脳の顆粒細胞でmGlur4 mRNAの強いまたは中程度の発現は検出されましたが、これらの細胞ではmGlur7 mRNAの有意な発現は見られませんでした。mGlur7 mRNAが激しくまたは適度に発現した他のニューロンまたは領域では、mGlur4 mRNAの有意な発現は観察されませんでした。これは、嗅球の僧帽弁と房細胞でした。前部嗅覚核;新皮質領域;帯状皮質;脳レトロス皮質;梨状皮質;周囲皮質;CA1;Ca3;歯状回の顆粒細胞;皮下皮質の表在層;胚性、麻痺、および前皮質の深層;外側中隔核の腹部;隔膜局所核;三角中隔核;対角線帯の核。末端の層核;淡い淡lidum;Claustrum;挿入された核以外の扁桃体核。撮影領域;内側乳腺核以外の視床下部核。腹側外側膝状核;遺伝子座coeruleus;Purkinje細胞;上脳炎以外の下脳幹の多くの核、末葉核核核核、橋核、および背側co牛核以外の核。脊髄の背部角。mGlur4 mRNAとmGluR7 mRNAの両方は、嗅覚結節、偏光皮質の表在層、Ca4、隔膜脳核、扁桃体の介入核、内側核核、多くのタラミン核および核nucleiおよび骨nucle核、および骨nucileおよび骨nucileおよび沈着した核で適度または激しく発現していました。mGlur4 mRNAとmGlur7 mRNAの両方の強い発現は、三叉神経節と背側根神経節でさらに検出されましたが、翼状虫神経節と上頸神経膠核でそれらの有意な発現は見つかりませんでした。結果は、グルタミン酸作動性神経系におけるL-AP4感受性の代謝性グルタミン酸受容体の異なる役割を示しています。

The distribution of mRNAs for metabotropic glutamate receptors, mGluR4 and mGluR7, which are highly sensitive for L-2-amino-4-phosphonobutyrate (L-AP4), was examined in the central nervous system of the rat by in situ hybridization. In general, the hybridization signals of mGluR7 mRNA were more widely distributed than those of mGluR4 mRNA, and differential expression of mGluR4 mRNA and mGluR7 mRNA was clearly indicated in some brain regions. Intense or moderate expression of mGluR4 mRNA was detected in the granule cells of the olfactory bulb and cerebellum, whereas no significant expression of mGluR7 mRNA was found in these cells. In other neurons or regions where mGluR7 mRNA was intensely or moderately expressed, no significant expression of mGluR4 mRNA was observed. Such were the mitral and tufted cells of the olfactory bulb; anterior olfactory nucleus; neocortical regions; cingulate cortex; retrosplenial cortex; piriform cortex; perirhinal cortex; CA1; CA3; granule cells of the dentate gyrus; superficial layers of the subicular cortex; deep layers of the entorhinal, parasubicular, and presubicular cortices; ventral part of the lateral septal nucleus; septohippocampal nucleus; triangular septal nucleus; nuclei of the diagonal band; bed nucleus of the stria terminalis; ventral pallidum; claustrum; amygdaloid nuclei other than the intercalated nuclei; preoptic region; hypothalamic nuclei other than the medial mammillary nucleus; ventral lateral geniculate nucleus; locus coeruleus; Purkinje cells; many nuclei of the lower brainstem other than the superior colliculus, periaqueductal gray, interpeduncular nucleus, pontine nuclei, and dorsal cochlear nucleus; and dorsal horn of the spinal cord. Both mGluR4 mRNA and mGluR7 mRNA were moderately or intensely expressed in the olfactory tubercle, superficial layers of the entorhinal cortex, CA4, septofimbrial nucleus, intercalated nuclei of the amygdala, medial mammillary nucleus, many thalamic nuclei, and pontine nuclei. Intense expression of both mGluR4 mRNA and mGluR7 mRNA was further detected in the trigeminal ganglion and dorsal root ganglia, whereas no significant expression of them was found in the pterygopalatine ganglion and superior cervical ganglion. The results indicate differential roles of the L-AP4-sensitive metabotropic glutamate receptors in the glutamatergic nervous system.